Figure 4. G6P hydrolysis in the presence of extravesicular Mg²⁺ or Mg²⁺ and EDTA in NMM (Fig. 4A) and MDM (Fig. 4B) permeabilized with taurocholic acid.

NMM and MDM were pretreated in ice for 15 min with 1% taurocholic acid. Microsomes were then incubated at the concentration of 0.3 mg protein/ml in the presence of 1mM Mg²⁺ or 1mM Mg²⁺ plus a concentration of EDTA sufficient to chelate all external Mg²⁺ [21] as described in the legend to Fig. 3. The incubation was carried out up to t = 30 min as indicated before. Data are means±S.E. of 6 different preparations. *Statistically significant vs. the corresponding sample in the absence of EDTA.