Figure 9.
PRE results mapped on the P protein crystal structure. The results of the NMR PRE experiments show two regions of P protein involved in the anion binding. The color of each residue indicates the [Cr(CN)6]3− concentration at which the associated resonance intensity is affected: red is 2.5 μM, orange is 5 to 10 μM, and yellow is 25 to 50 μM. Residues colored gray correspond to resonances already missing in the 0M [Cr(CN)6]3− HSQC spectrum, prolines or unresolved peaks. Residues colored green are unaffected by [Cr(CN)6]3− between 2.5 μM and 100 μM concentration range.