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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Brain Behav Immun. 2011 Feb 18;25(4):787–799. doi: 10.1016/j.bbi.2011.02.007

Fig. 4.

Fig. 4

CGRP inhibits the expression of LPS-induced chemokines at the mRNA level. HMEC-1 cells (1.5 ×106 cells) were stimulated with LPS (1 μg/ml) in the presence or absence of CGRP (10 nM) for 12 h. Total RNA was extracted and transferred onto nylon membranes. The expression of CXCL8, CCL2, and CXCL1 mRNA in HMEC-1 cells was analyzed by Northern blotting. This experiment was performed 4 times and the result illustrated by this figure was seen in all 4 experiments.