(A). The expressions of phospho-Akt (p-Akt), Akt, p-p70S6K, p70S6K, p-eIF4E, and eIF4E were analyzed by Western blottingin control (IRES) and RASSF1A (RF1A) cells. (B). The expressions of myc, p-Akt, p-ASK1, p-p38, p38, p-eIF4E, eIF4E, Bcl2 and cyclin D1 were determined by Western blotting in RASSF1A cells transfected with either empty (-) or Akt1-expressing vector (+). α-tubulin was used as an internal control.A single representative from three independent experiments is shown.