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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Brain Behav Immun. 2011 Jan 13;25(4):629–639. doi: 10.1016/j.bbi.2010.12.016

Figure 5. Mixed glial cultures robustly upregulate concentrations of secreted TNF and CCL2 when exposed to putrescine and spermine, but not spermidine.

Figure 5

0μM 1μM, 100μM, or 1000μM concentrations of putrescine, spermidine, or spermine were added to mixed glial cultures in the absence of FBS for 24 hours. Concentrations of TNF (A) and CCL2 (B) (**P < 0.01, ***P < 0.001, two-tailed Student's t-test).