Figure 8. High concentrations of all three polyamines decreased the percentage of oligodendrocytes in culture.
0μM 1μM, 100μM, or 1000μM concentrations of putrescine, spermidine, or spermine were added to mixed glial cultures in the absence of FBS for 24 hours. Total number of cells per slide chamber (A) and percentage of CNP+ cells per slide chamber (B) (*P < 0.05, ***P < 0.001, two-tailed Student's t-test).