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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Immunity. 2011 Mar 31;34(4):590–601. doi: 10.1016/j.immuni.2011.02.016

Fig. 3. Monocyte emigration is CCR2-dependent and is mediated by MCP1 produced by non-hematopoietic cells.

Fig. 3

(A and B) Fixed-frozen bone marrow sections from naïve and LPS-treated (2ng/mouse) CCR2 reporter mice (A) and CCR2 reporter-Ccr2−/− mice (B) were stained with Goat anti-mouse VE-cadherin and counterstained with Hoechst (63X). (C) Quantification of exiting monocyte percentage from CCR2 reporter and CCR2 reporter-Ccr2−/− mice following LPS treatment. Exiting monocyte percentages were calculated by dividing the total number of total GFP+ cells by the number of GFP+ cells within one cell distance of VE-cadherin+ endothelium. (E) Ccr2.GFP>WT and Ccr2.GFP>Ccl2−/− bone marrow chimeric mice were treated with 2ng/mouse LPS for 4hr. Fixed-frozen femurs were stained with anti-VE-cadherin. (F) Circulating monocyte frequencies in blood were determined in bone marrow chimeric mice. (G) The percentage of monocytes associated with VE-cadherin expessing endothelials, defined as exiting monocytes, in bone marrow was calculated. Data are representative of three independent experiments. Error bars indicate SEM. Scale bars indicate 90μm.