Fig. 6. MCP1 produced by Nestin-expressing MSCs is required for optimal monocyte emigration after LPS stimulation.
Ccl2-RFPflox/flox, Ccl2-RFPflox/flox expressing Nes-Cre or Tek-Cre, and Ccl2−/− were inoculated with LPS. 4 hr post stimulation, (A) bone marrow was sectioned and stained. Scale bars indicate 40μm. (B) blood was obtained analyzed by flow cytometry to determine CD11b+Ly6Chi monocyte frequencies in the circulation. The emigration kinetics of CD11b+Ly6Chi monocytes and CD11b+Ly6G+ neutrophils are shown in (C) and (D). Error bars indicate SEM.