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. 2011 Mar 24;34(4):579–589. doi: 10.1016/j.immuni.2011.02.015

Figure 4.

Figure 4

Lack of CD94 Results in Defective NK Cell Control of ECTV Spread

(A) B6 mice (gray columns) and B6.D2-D6 mice (white columns) were infected with ECTV, and virus titers in spleen and liver were determined 3 dpi. Data correspond to the average ± SD of five individual mice and are representative of two similar experiments.

(B) Wild-type B6 mice or CD94-deficient mice were depleted or not of NK cells with 15 μl anti-Asialo GM antibody and infected with ECTV. Virus titers in spleen (gray bars) and liver (white bars) were determined 3 dpi. Data correspond to the average ± SD of five individual mice from two independent experiments.

(C) Wild-type B6 mice treated with blocking 20D5HCmIgG1-Q mAb (gray columns) or an isotype-matched control mAb (white columns) were infected with ECTV and virus titers determined 3 dpi. Data correspond to the average ± SD of six individual mice from two independent experiments.

(D) Wild-type and CD94-deficient B6 mice were infected with ECTV. NK cell proliferation was determined 5 dpi in the spleen by BrdU incorporation. Bar graph is the cumulative data for three similar experiments with three mice/group. Representative flow cytometry plots of gated DX5+CD3− NK cells are shown on the right.

(E) Wild-type B6 and CD94-deficient mice were infected with ECTV or not and the draining popliteal lymph node was analyzed by flow cytometry 2 dpi. Shown are the cumulative data for five independent experiments as a column graph and representative plots indicating the proportion of NK cells (DX5+, CD3−) gated on lymphocytes as determined by FSC and SSC.

(F) Column graphs show the cumulative data for GzB- and IFN-γ-positive cells in the DX5+CD3− NK cell gate (from D) for five independent experiments. Representative flow cytometry plots as used for the columns graphs are shown on the right.

(G) B6 Wild-type or Qa-1b-deficient B6 mice depleted or not of NK cells with NK1.1 mAb treatment were infected with ECTV and the virus titers in the liver and spleen were determined 3 dpi. Data correspond to the mean ± SD of five mice (bars as indicated).