Figure 5.

Upregulation of NKG2E and Qa-1b during ECTV Infection

(A) Wild-type B6 mice were infected with ECTV in the footpad or not and the expression of NKG2A and NKG2 in the draining popliteal lymph node was determined. Flow cytometry plots correspond to pools of LNs from three mice and are representative of two similar experiments. Data show cells gated on NK1.1⁺CD3⁻ NK cells.

(B) As in (A) but showing histograms to highlight changes in mean fluorescence intensities (shaded histogram, uninfected mice; black line, infected mice). The numbers indicate the mean fluorescence intensity of the closest histogram.

(C) LN cells were magnetically sorted into DX5⁺ (NK cells, 40% pure) and DX5⁻ (non-NK, >99% pure). RNA was isolated, reverse transcribed, and the NKG2E message was quantified by qPCR with a specific primers/probe set. Data correspond to the mean ± SD of two wells and are representative of two similar experiments.

(D) RNA was obtained from the LNs of the indicated mice and analyzed by qPCR as in (C). Data correspond to the mean ± SD of two wells and are representative of two similar experiments.

(E) L cells were infected for 4 hr with 1 PFU/cell of sucrose-purified ECTV virus. Surface expression of Qa-1 was analyzed by flow cytometry. Data are representative from three similar experiments (shaded gray, isotype-matched control Ig; thin line, anti-Qa-1 staining of uninfected L cells; thick line, anti-Qa-1 staining of infected L cells).