. Author manuscript; available in PMC: 2012 May 15.

Published in final edited form as: Arch Biochem Biophys. 2011 Feb 19;509(2):186–190. doi: 10.1016/j.abb.2011.02.006

TABLE 1. Fluorescence Recovery After Photobleaching Results of PLCβ1 enzymes expressed in HEK293 Cells.

	MobileFraction	t_1/2 (sec)	n
eYFP-PLCβ₁	69 ± 9%	8.5±1.8	5
eYFP-LCβ₁S887A	61 ± 8%	10.6±2.7	6
eYFP-PLCβ1S887D	60 ± 9%	9.7±1.9	6

where enzyme was transiently transfected in HEK293 cells and viewed using L15 medium. In this study, a 2 μ spot was bleached by a 100mW laser and the recovery curves were fit to a single exponential. The mobile fraction represents the percent of the fluorescence that recovers as compared to a spot in the cell that was not bleached. The value of t _1/2 is the midpoint of the recovery.