Table 4.
Isotopomer distribution of glutamate in RBCs incubated with [13C1,2,3,4] α-ketoglutarate, alanine, and glucose
Time (h) | Percent Mass isotopomer distribution (glutamate)a | ||||
---|---|---|---|---|---|
Monoisotopic | M + 1 | M + 2 | M + 3 | M + 4 | |
0 | 95.3 ± 1.7 | 4.66 ± 1.66 | 0 | 0 | 0 |
6 | 15.9 ± 0.7** | 0.72 ± 0.08** | 0.10 ± 0.10 | 2.52 ± 0.37* | 80.8 ± 0.3** |
12 | 10.4 ± 0.7** | 0.41 ± 0.01** | 0 | 3.08 ± 0.55* | 86.1 ± 0.9** |
21.25 | 7.47 ± 0.24** | 0.24 ± 0.01** | 0 | 2.82 ± 0.14* | 89.4 ± 0.3** |
Expected distribution due to natural abundance | 93.40 | 5.64 | 0.91 | 0.05 | 0.00 |
aMeans and S.E.M. for n = 3
* p < 0.05; ** p < 0.001