Table 5.
Isotopomer distribution of GSH in RBCs incubated with [13C1,2,3,4] α-ketoglutarate, alanine, and glucose
Time (h) | Percent Mass isotopomer distribution (GSH)a | ||||
---|---|---|---|---|---|
Monoisotopic | M + 1 | M + 2 | M + 3 | M + 4 | |
0 | 85.6 ± 2.5 | 11.4 ± 2.9 | 1.47 ± 0.86* | 1.59 ± 0.81 | 0 |
6 | 86.6 ± 1.1 | 7.99 ± 0.19* | 1.67 ± 0.45* | 0.33 ± 0.33 | 3.42 ± 0.33** |
12 | 80.9 ± 0.8 | 8.08 ± 0.60 | 2.11 ± 0.34* | 0.80 ± 0.42 | 8.09 ± 0.15** |
21.25 | 78.4 ± 0.9* | 7.96 ± 0.67 | 1.03 ± 0.52* | 1.38 ± 0.18* | 11.3 ± 1.2* |
Expected distribution due to natural abundance | 82.93 | 10.90 | 5.43 | 0.62 | 0.10 |
aMeans and S.E.M. for n = 3
* p < 0.05; ** p < 0.001