. 2010 Dec 27;22(5):613–624. doi: 10.1089/hum.2010.241

Table 2.

Integrated Rep Gene Copy Numbers in 293-Based Cell Lines and Vector Yields^a

Cell line	Vector produced	Rep copies/cell	Purified vector (vg) from 20 × 15-cm plates
293-ssAAV2-GFP-145	ssAAV2-CMV-GFP	34 ± 1.63	2.8 × 10¹³
293-dsAAV2-GFP-12	dsAAV2-CMV-GFP	17 ± 4.74	2.0 × 10¹³
293-dsAAV8-GFP-23	dsAAV8-CMV-GFP	28 ± 3.56	1.2 × 10¹³
293-dsAAV9-GFP-34	dsAAV8-D(CMV)-GFP	23 ± 3.23	1.5 × 10¹³
293-ssAAV9-Dys3978-14	dsAAV9-CMV-hDys3978	36 ± 5.25	2.5 × 10¹³

^{^a}

The copy numbers displayed in the table were those in the stable cell lines before Ad-Cre infection. SYBR Green–based real-time PCR methods were utilized here (see Material and Methods for details).