Table 5.

Binding of CD176 antibody in sandwich ELISA with catcher antibody: CD44

Tissue derivation	Cell lines	CD176+ on CD44+
Lung cancer
Adenocarcinoma	SPC-A-1	+
Adenocarcinoma	GLC-82	+
Small cell carcinoma	NCIH446	(+)
Giant cell carcinoma	801-D	(+)
Breast cancer
Adenocarcinoma	MDA-231	−
Adenocarcinoma	MDA-435	+
Adenocarcinoma	MCF-7	(+)
Liver cancer
HCC	HepG-2	+
HCC	HuH-7	(+)

Score according to OD₄₉₂ values minus blank as follows: +, >0.2; (+), 0.1–0.2; −, <0.1.

HCC, hepatocellular carcinoma.