Skip to main content
. 2011 Feb 3;20(4):670–683. doi: 10.1002/pro.596

Figure 1.

Figure 1

Size exclusion profiles and circular dichroism. A: Size exclusion elution profiles for wild-type MGL 1-303, red dotted line, and mutant MGL (hMGL 1–303 Leu169Ser, Leu176Ser), black solid line, purified in the absence of detergent showing 100% aggregation and 90% monomer, respectively. B: Circular dichroism structural analysis of wild-type MGL 1-303, red line, and mutant MGL (hMGL 1–303 Leu169Ser, Leu176Ser), black line. (i) Far UV scans from 200 to 260 nm. (ii) Temperature melts from 25 to 80C monitored at 210 nm.