Table I.
Monoglyceride Lipase Constructs Generated and Purification Yields* in mg/L
| Yield mg/L | |
|---|---|
| Lid-domain mutations | |
| 1-303 Leu169Ser, Leu176Ser | 4.5 |
| 1-303 Leu167Gln | 2.3 |
| 1-303 Leu171Gln | 0.7 |
| 1-303 Leu174Gln | 0 |
| 1-303 Leu167Gln, Leu171Gln | 5 |
| 1-303 Leu167Gln, Leu174Gln | 7 |
| 1-303 Leu171Gln, Leu174Gln | 1.5 |
| 1-303 Leu167Gln, Leu171Gln, Leu174Gln | 0 |
| Lid-domain mutations + truncations | |
| 1-297 Leu169Ser, Leu176Ser | 2 |
| 1-292 Leu169Ser, Leu176Ser | 0.5 |
| 9-303 Leu169Ser, Leu176Ser | 2 |
| 9-297 Leu169Ser, Leu176Ser | 1 |
| 19-303 Leu169Ser, Leu176Ser | 0 |
| 19-297 Leu169Ser, Leu176Ser | 0 |
| 19-292 Leu169Ser, Leu176Ser | 0 |
| 26-303 Leu169Ser, Leu176Ser | 0 |
| 26-297 Leu169Ser, Leu176Ser | 0 |
| 26-292 Leu169Ser, Leu176Ser | 0 |
| 33-303 Leu169Ser, Leu176Ser | 0 |
| 33-297 Leu169Ser, Leu176Ser | 0 |
| 33-292 Leu169Ser, Leu176Ser | 0 |
| Lid-domain mutations + Surface mutations | |
| 1-303 Leu169Ser, Leu176Ser, Lys36Ala | 3.6 |
| 1-303 Leu169Ser, Leu176Ser, Lys160Ala | 0.7 |
| 1-303 Leu169Ser, Leu176Ser, Lys165Ala | 0.5 |
| 1-303 Leu169Ser, Leu176Ser, Lys226Ala | 2.3 |
| 1-303 Leu169Ser, Leu176Ser, Lys36Ala, Lys236Ala | 1.5 |
*
Purification yields are from one single experiment.