Table I.
Monoglyceride Lipase Constructs Generated and Purification Yields* in mg/L
Yield mg/L | |
---|---|
Lid-domain mutations | |
1-303 Leu169Ser, Leu176Ser | 4.5 |
1-303 Leu167Gln | 2.3 |
1-303 Leu171Gln | 0.7 |
1-303 Leu174Gln | 0 |
1-303 Leu167Gln, Leu171Gln | 5 |
1-303 Leu167Gln, Leu174Gln | 7 |
1-303 Leu171Gln, Leu174Gln | 1.5 |
1-303 Leu167Gln, Leu171Gln, Leu174Gln | 0 |
Lid-domain mutations + truncations | |
1-297 Leu169Ser, Leu176Ser | 2 |
1-292 Leu169Ser, Leu176Ser | 0.5 |
9-303 Leu169Ser, Leu176Ser | 2 |
9-297 Leu169Ser, Leu176Ser | 1 |
19-303 Leu169Ser, Leu176Ser | 0 |
19-297 Leu169Ser, Leu176Ser | 0 |
19-292 Leu169Ser, Leu176Ser | 0 |
26-303 Leu169Ser, Leu176Ser | 0 |
26-297 Leu169Ser, Leu176Ser | 0 |
26-292 Leu169Ser, Leu176Ser | 0 |
33-303 Leu169Ser, Leu176Ser | 0 |
33-297 Leu169Ser, Leu176Ser | 0 |
33-292 Leu169Ser, Leu176Ser | 0 |
Lid-domain mutations + Surface mutations | |
1-303 Leu169Ser, Leu176Ser, Lys36Ala | 3.6 |
1-303 Leu169Ser, Leu176Ser, Lys160Ala | 0.7 |
1-303 Leu169Ser, Leu176Ser, Lys165Ala | 0.5 |
1-303 Leu169Ser, Leu176Ser, Lys226Ala | 2.3 |
1-303 Leu169Ser, Leu176Ser, Lys36Ala, Lys236Ala | 1.5 |
Purification yields are from one single experiment.