Figure 3.

¹H-¹⁵N HSQC NMR spectra of Mg²⁺-bound forms of CaMC wild type and DD mutant. (A) HSQC spectra of CaMC wild type (left) and DD mutant (right) with 100 mM Mg²⁺ at 50°C. I130, V136, N137, and Y138 are labeled. (B) Secondary shifts of I130, V136, N137, and Y138 calculated with Cα (left) and CO (right) chemical shifts. Open bars correspond to the shift of the apo form of the wild type, blue bars represent the shift of the Mg²⁺-bound form of the wild type and green bars indicate the shift of the Mg²⁺-bound form of the DD mutant. Asterisks show residues with uncalculated secondary shifts, due to the lack of assignment.