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Effects of I/R on PKN Thr774 phosphorylation. (A) Time course of PKN Thr774 phosphorylation. (B) The level of PKN Thr774 phosphorylation was determined by densitometric analysis. The mean total PKN/actin or phosphorylated PKN/total PKN in sham operated mouse hearts was set as 1 (dotted line). n=3–4. * p<0.05 vs. sham
