Fig. 4. I/R injury was exacerbated in DN-PKN Tg mice.
Tg-DNPKN and NTg mice were subjected to I/R. (A) Expression of transgene (DNPKN, PKN K664D) is shown. (B) Gross appearance of LV tissue sections after Alcian blue and TTC staining in Tg-DNPKN and NTg mice subjected to I/R. (C, D) The quantitative analyses of AAR/LV size (%) (C) and MI area/AAR (%) (D) are shown. (E, F) LV tissue sections were subjected to TUNEL and DAPI staining. (E) Representative stainings with TUNEL and DAPI. (F) The number of TUNEL-positive nuclei was expressed as a percentage of total nuclei detected by DAPI staining. *p<0.05, **p<0.01 vs NTg.