Fig. 7. Proteasome is activated in Tg-CAPKN mouse hearts.
Chymotrypsin-like peptidylglutamyl-peptide hydrolase activity of proteasomes present in the cytoplasmic milieu was evaluated with the fluorogenic peptide LLVY-MCA. (A) Chymotrypsin-like activity in either Tg-CAPKN or NTg hearts was evaluated. The mean proteasome activity in NTg was set as 1. *p<0.05 vs. NTg. (B and C) Chymotrypsin-like activity in the heart subjected to I/R was measured in Tg-CAPKN (B) or Tg-DNPKN mice (C) and compared with that in NTg mice. The mean proteasome activity in NTg-Sham hearts was set as 1. N=4. (D) Epoxomicin (Epo. 0.5 mg/kg) or vehicle (Veh.) was injected intraperitoneally in Tg-CAPKN or NTg mice one day before and 1 hour before coronary occlusion. Mice were then subjected to I/R. Infarct size (% of AAR) are shown. The values of AAR were not different between all groups (not shown).