Skip to main content
. Author manuscript; available in PMC: 2011 Oct 21.
Published in final edited form as: Nature. 2011 Apr 21;472(7343):361–365. doi: 10.1038/nature09976

Figure 1. TRIM5 promotes innate immune signaling.

Figure 1

a –c, HEK-293 cells transfected with the indicated pcDNA-based expression plasmids and luciferase reporters for AP-1 (a), NF-κB (b) or IFNB1 (c). Bars show mean luciferase activity +/− s.d. (n = 6). d, Global expression profile comparing TRIM5 KD to control KD THP-1 macrophages. Triangles indicate inflammatory genes significantly downregulated in TRIM5 KD. e, qRT-PCR for the indicated mRNAs harvested 2 to 8 hrs after LPS-treatment, depending on the peak values for that gene. Shown are the means +/− s.e.m (n = 3) relative to untreated cells. f, Concentration of the indicated proteins in the culture supernatant, 24 hrs after LPS-treatment (mean +/− s.d., n = 3). RNA and protein data are representative of at least 3 separate donors. g–j, THP-1 macrophages transduced with miR30-based lentivirus KD vectors targeting either TRIM5 (g and i), IRF3 (h), or STAT2 (i), were treated 24 hrs with the indicated compounds and challenged with VSV-G pseudotyped HIV-1 luciferase reporter virus (g–i) or with the indicated GFP reporter viruses (j). Data are expressed as fold-change compared to control KD cells, with s.e.m (n = 4). All data are representative of at least 3 independent experiments.