Figure 2.
Leptin (L) inhibits serum-stimulated H4IIE cell proliferation in vitro. H4IIE hepatocellular carcinoma cells were cultured in medium containing either 0.1% (v/v) foetal bovine serum (FBS) (LSM) or 1.0% (v/v) FBS in the absence or presence of leptin (L, 100 ng/ml). Number of cells was calculated as a fold change vs. number of cells at day 0, n = 6 separate experiments; *P < 0.05 1.0% (v/v) FBS + L vs. 1.0% (v/v) FBS