Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 24;74(4):567–573. doi: 10.1021/np100429s

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011 American Chemical Society and American Society of Pharmacognosy and American Society of Pharmacognosy

This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.

PMC Copyright notice

Inhibition of CYP or hydrogenation of austocystin D abrogates austocystin D DNA damage and cytotoxicity. (a) Inhibitory effect of the CYP inhibitor ketoconazole on HCT-15 cell growth inhibition of 0.5 μM (light blue), 1 μM (blue), and 2 μM (dark blue) austocystin D. (b) Inhibitory effect of 10 μM ketoconazole on austocystin D-induced phosphorylation of histone H2AX in HCT-15 cells following 1 h ketoconazole pretreatment and 4 h austocystin D treatment. (c) Specific inhibition of 10 μM ketoconazole (Ket) on 10 μM austocystin D-induced phosphorylation of histone H2AX in SW620 cells in comparison to 20 μM camptothecin. (d, e) Comparison of austocystin D and dihydro-austocystin D for (d) growth inhibition after 72 h treatment of MCF7 cells and (e) induction of phosphorylation of histone H2AX after 4 h in MCF7 cells. Vertical bars represent SEM.