Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady state kinetic measurements on wild-type HPAO and mutants.a
| Enzyme Form |
kcat (s−1) | kcat/Km[MeA] (µM−1 s−1) | kcat/Km[O2](µM−1 s−1) |
|---|---|---|---|
| Wt | 6.2 ± 0.5 | 0.18 ± 0.04 | 0.80 ± 0.10 |
| L425F | 4.9 ± 0.1 | 0.15 ± 0.02 | 0.077 ± 0.007 |
| I622Y | 5.3 ± 0.1 | 0.091 ± 0.01 | 0.69 ± 0.10 |
| I639F | 5.3 ± 0.1 | 0.17 ± 0.02 | 1.2 ± 0.2 |
| L643F | 4.5 ± 0.4 | 0.13 ± 0.02 | 0.76 ± 0.12 |
All measurements carried out at pH 8 and 25°C.
