Figure 2. 17β-estradiol and equol modulation of glucose uptake and glycolysis.
Western blot analysis of Glut-4 (A), GAPDH (B), and PDHe1α (C) expression was performed on NHP hippocampal samples from both of the hormone treatment paradigms as well as controls. Expression levels for each sample were normalized to beta-actin levels (Glut-4) and beta-tubulin levels (GAPDH and PDHe1α). Expression levels were then normalized to the control animals (controls were set to 100%). Statistically significant differences were calculated using a two-tailed, one-way analysis of variance (ANOVA) followed by a Bonferroni post-hoc correction. Hormone treatment did not significant affect expression levels of these three proteins in the NHP hippocampus. Bars represent % control ± S.E.M., n = 8 for each condition.