Skip to main content
. Author manuscript; available in PMC: 2011 Apr 25.
Published in final edited form as: J Am Coll Cardiol. 2010 Apr 6;55(14):1476–1486. doi: 10.1016/j.jacc.2009.11.066

Figure 8. Cultured Cardiac Myocytes.

Figure 8

AC6 knockdown by specific small interfering ribonucleic acid (siRNA) followed by phenylephrine stimulation (10 µmol/l, 16 h) reduced mRNA expression (quantitative reverse transcriptase-polymerase chain reaction) of AC6 (A), ANF (B), and β-MHC (C) and also reduced FHL1 protein expression (Western blotting) in cultured cardiac myocytes (G) Cultured cardiac fibroblasts. AC6 knockdown reduced mRNA expression of AC6 (D), collagen type Iα1 (E), and collagen type IIIα1 (F) and also reduced periostin protein expression (Western blotting) in cultured cardiac fibroblasts (H). p Values above bars derived from Student t test (2-tailed) versus same condition for CON. Error bars = 1 SE. Data in A through F are from 3 independent experiments. KD = AC6 knockdown; other abbreviations as in Figures 1, 2, 4, and 6.