Table 1. Morpholino knockdown of col8a1 recapitulates the notochord distortion observed in gulm208 mutants.
Wild-type embryos were injected with morpholino and live embryos sorted to new dishes at 10 hpf; these embryos were scored for notochord distortion at 30 hpf. Data shown are the pooled results of three independent experiments.
| Specific Morpholino | Dose of morpholino (ng) | Embryos scored (#) | Dead or dysmorphic embryos (#) | Notochord distortion | |
|---|---|---|---|---|---|
| - | + | ||||
| Control | 12 | 186 | 10 | 172 (98%) | 4 (2%) |
| col8a1 (splice) | 2.4 | 170 | 2 | 2 (1%) | 166 (99%) |