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. Author manuscript; available in PMC: 2011 Apr 25.
Published in final edited form as: J Neurochem. 2008 Jan 11;107(6):1709–1721. doi: 10.1111/j.1471-4159.2008.05737.x

Table 1.

Comparative characteristics of amperometric events recorded from chromaffin cells treated with 10 μM METH. The median values of each parameter were first calculated for amperometric events recorded from individual cells and then the average of the medians was computed. The data are presented as percentages of changes in amperometric spike characteristics relative to matched controls recorded from sister cultures at the same day (N = 85 cells for control, 30 cells for 1h METH, 72 cells for 24h METH and 15 cells for 48 h METH). For absolute values of spike parameters in control cells and those treated with 10 μM METH for 24 h see Supplementary Table 1.

Inter-spike interval Events/stimulation t1/2 Imax QN trise (25–75%) Rise slope Fall τ1 Fall τ2
1h METH 112 ± 17 106 ± 11 136 ± 9*# 38 ± 3* 59 ± 4* 168 ± 15* 26 ± 5* 216 ± 16* 164 ± 26*
24h METH 111 ± 16 111 ± 7 102 ± 8 143 ± 9* 126 ± 5* 98 ± 5 203 ± 26* 95 ± 9 102 ± 6
48h METH 91 ± 13 90 ± 3 94 ± 14 148 ± 29* 146 ± 17* 102 ± 9 173 ± 69* 82 ± 14 114 ± 19
#

Although t1/2 was significantly increased in cells acutely treated with METH, this might be due to several reasons, including alteration of the fusion pore kinetics or the composition of the intravesicular matrix. It is also possible that smaller amperometric spikes are more affected by the noise and thereby have longer t1/2 due to artifact (Mosharov and Sulzer 2005).

*

p < 0.05 versus untreated cells.