Fig. 1.
The duration of anoxic viability decreases with developmental stage. A: Embryos were placed into anoxia at 4 hpf (triangles), 24 hpf (squares) or 48 hpf (circles) at 28.5° C, returned to normoxia at intervals, and the percentage that had resumed development 24 h later was determined. Error bars are ±SEM. B: The duration of anoxia after which half of the embryos were no longer viable when returned to normoxia (open circles) plotted versus the developmental age at which the embryos were placed into anoxia, with best-fit line (slope = -1.02, R2 = 0.96).