Fig. 2.

Duration of anoxic viability can be reset and varies with temperature. A: Embryos were allowed to develop to 24 hpf in normoxia (N) and were then placed into anoxia (A) for 24 h, removed to normoxia for 4 h, and finally returned to anoxia. The viability at time points of this second anoxic exposure was assessed (closed triangles). The dashed line indicates the 52 hpf threshold of anoxic viability observed for a first anoxic exposure at which time the embryos had only completed 28 h of normoxic development. B: Embryos were placed into anoxia at 24 hpf at three different temperatures, returned to normoxia at intervals, and the percentage recovering after 24 more hours was determined. Error bars are ±SD.