Fig. 4.

Anoxic arrest is mimicked by chemical inhibition of oxidative phosphorylation. A: Schematic of the electron transport chain indicating the sites of action of mitochondrial inhibitors. B: Images comparing embryos incubated in anoxia or various inhibitors for 24 h, and then the same embryos after 24-48 h of recovery after a return to normoxia or washing of the chemical inhibitor. C: The percent survival after 24 h of anoxia or treatment with chemical inhibitors beginning at 24 hpf and the percent recovery after an additional 24 h was measured. A, anoxia; R, rotenone; M, myxothiazole; O, oligomycin. D: Rate of lactate accumulation in 24 hpf embryos after 8 h in different mitochondrial inhibitors or anoxia. E: 4 hpf embryos were incubated for 1 h in 10 μM oligomycin, were washed or left in the drug for 17 additional hours, and the rate of lactate accumulation was determined. F: Embryos at 5 hpf placed into varying doses of KCN were incubated for 24 h after which the relative rate of growth was determined as described in Experimental Procedures. The best-fit line describes an exponential decline (R² = 0.99). All experiments were performed at 24° C. All error bars are ±SD.