Fig. 5.

The AMP-activated protein kinase (AMPK) pathway is activated by the inhibition of oxidative phosphorylation in zebrafish embryos. A: Immunoblot analysis of AMPK phosphorylation before and after 2 min in KCN, with increasing KCN doses, or with increasing time in anoxia. B: Phosphorylation of Acetyl-CoA Carboxylase (ACC) with time in KCN or anoxia. C: Phosphorylation of p70-S6 Kinase (S6K) in KCN or anoxia. D: 72 hpf embryos were incubated in KCN and the timing of AMPK phosphorylation was assessed. E: Change in the AMP/ATP ratio after a 5 min inhibition of oxidative phosphorylation as described in Experimental Procedures. Error bars are ±SD. All experiments utilized 24 hpf embryos.