Table 2.
Biochemical effects of root peel-extract of P. fulgens and PZQ on test parasites in vitro
| Treatment (mg/ml) | Enzyme Activities (total/specific*) | Decrease (%) | ||||
|---|---|---|---|---|---|---|
| AcPase | AlkPase | ATPase | AcPase | AlkPase | ATPase | |
| R. echinobothrida | ||||||
| Control (PBS) |
17.83 ± 0.28 1.43 ± 0.03* |
126.01 ± 1.53 10.25 ± 0.16* |
232.42 ± 3.32 11.19 ± 0.16* |
|||
|
P. fulgens (20 mg/ml) |
5.49 0.50 ± 0.01* ± 0.05 |
42.30 ± 0.81 3.57 ± 0.13* |
163.54 ± 12.58 8.61 ± 0.68* |
69.20 | 66.43 | 29.63 |
| P < 0.0001 | P < 0.0001 | P < 0.05 | ||||
| PZQ (0.02 mg/ml) |
6.72 ± 0.23 0.59 ± 0.03* |
34.87 ± 0.78 3.15 ± 0.09* |
184.80 ± 1.87 9.58 ± 0.11* |
62.31 | 72.33 | 20.48 |
| P < 0.0001 | P < 0.0001 | P > 0.0001 | ||||
| G. crumenifer | ||||||
| Control (PBS) |
108.94 ± 0.54 5.39 ± 0.03* |
174.46 ± 1.78 8.05 ± 0.10* |
153.31 ± 7.10 7.48 ± 0.35* |
|||
|
P. fulgens (20 mg/ml) |
56.69 ± 2.58 3.27 ± 0.15* |
84.10 ± 5.27 4.13 ± 0.26* |
87.95 ± 2.80 5.02 ± 0.17* |
47.96 | 51.79 | 42.63 |
| P < 0.0001 | P < 0.0001 | P > 0.001 | ||||
| PZQ (0.02 mg/ml) |
52.03 ± 2.23 3.07 ± 0.18* |
79.43 ± 1.21 3.83 ± 0.07* |
103.58 ± 10.48 6.03 ± 0.63* |
52.24 | 54.47 | 32.43 |
| P < 0.0001 | P < 0.0001 | NS | ||||
Values are given as mean (SEM) from three replicates (n = 3)
Total activity formation of 1 μmol of product/h/g of wet tissue
* Specific activity activity/mg protein
P < 0.05, statistically significant; P < 0.0001, extremely significant; NS not significant