Skip to main content
. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: Cell Metab. 2010 May 5;11(5):390–401. doi: 10.1016/j.cmet.2010.03.014

Figure 2. TSC2 is not required for downregulation of S6K1 T389 by energy-depleting agents.

Figure 2

(A) TSC2+/+ or TSC2-/- MEFs were treated with either 10μM oligomycin or (B) 100mM 2DG for the times indicated. (C) TSC2+/+ or TSC2-/- MEFs were treated with the indicated concentrations of 2DG for 30min. (D) Western blot analysis of the indicated proteins in TSC2+/+ and TSC2-/- MEFs. (E and F) TSC2+/+ MEFs were treated with either 10μM oligomycin or 25mM 2DG at the indicated times. * denotes the addition of a fresh aliquot of either ATP-depleting agent following a 60min incubation with the initial inhibitor. The data shown are representative of at least three independent experiments.