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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: Cell Metab. 2010 May 5;11(5):390–401. doi: 10.1016/j.cmet.2010.03.014

Figure 6. Absence of AMPK does not affect metformin-mediated glucose uptake.

Figure 6

(A) L6 myotubes were transfected with either non-silencing dsRNA (NS) or with two different dsRNAs directed against either the α1 or the α2 catalytic subunit of AMPK and incubated for 72 hrs. Cells were then treated with either 5mM metformin for the last 24 hrs or 2mM AICAR for the last 2 hrs. Parallel plates were either extracted for western blotting with the indicated antibodies or (B) further processed for glucose-uptake measurements, as described in Experimental Procedures. Results are representative of three independent experiments conducted in triplicate.