Table 1. Primers for PCR amplification of exons of candidate genes and the size of the PCR products.
|
Gene |
Exon |
Primer direction |
Primer sequence (5′-3′) |
Fragment size (bp) |
Gene |
Exon |
Primer direction |
Primer sequence (5′-3′) |
Fragment size (bp) |
|
CRYAA |
1 |
F |
acttgtcccagccacgttt |
515 |
|
3 |
F |
ttcccggtatgtcctagcag |
511 |
| |
|
R |
ctctgcaaggggatgaagtg |
|
|
|
R |
ctggtgtctcaatccccaac |
|
| |
2 |
F |
aaccagccacctgaccatag |
637 |
|
4 |
F |
atcaaccagctttggaggaa |
527 |
| |
|
R |
acattagctcgggaatggtg |
|
|
|
R |
cttgcagtgagctgagatcg |
|
| |
3 |
F |
caggggcatgaatccataaa |
551 |
|
5 |
F |
acggtgttgagtgtgaatgg |
679 |
| |
|
R |
taagctctcctggctgctct |
|
|
|
R |
ggctctgcctgaaaggatta |
|
|
CRYBA1/A3 |
1 |
F |
gggcctctctggatttctgt |
404 |
CRYGC |
1 |
F |
tttccagtgaatgcaggatg |
672 |
| |
|
R |
gctagggcagtggttattgc |
|
|
|
R |
tctgctgtttttgtgcatgtt |
|
| |
2 |
F |
gcagaggttgcagtgaagtg |
516 |
|
2 |
F |
cgcagcaaccacagtaatct |
641 |
| |
|
R |
caatggcatccacagtcatc |
|
|
|
R |
caacgtctgaggcttgttca |
|
| |
3 |
F |
actctgggcaaatgaacacc |
547 |
CRYGD |
1 |
F |
gagagaatgcgaccaaaacc |
742 |
| |
|
R |
tctttatccagcccctgaaa |
|
|
|
R |
gcttatgtggggagcaaact |
|
| |
4 |
F |
cctgtcaactcattcctcaactc |
557 |
|
2 |
F |
tgtgctcggtaatgaggagtt |
586 |
| |
|
R |
tgggctcttgagtatccactt |
|
|
|
R |
cacatcttggttgccatttg |
|
| |
5 |
F |
tggttggctgcattttgtta |
609 |
CRYGS |
1 |
F |
tgtctgggttttggtttcttg |
480 |
| |
|
R |
gcatgtctgggggagataaa |
|
|
|
R |
cggtaattctgttaagggtgga |
|
| |
6 |
F |
cccccagacatgcctctat |
648 |
|
2 |
F |
tgcttcatgttcagccttca |
662 |
| |
|
R |
ttacactccagcctgagcaa |
|
|
|
R |
ctcaagaggcagagacagca |
|
|
CRYBB2 |
1 |
F |
cagaggggagtggtctcaag |
540 |
|
3 |
F |
gtgctgtctctgcctcttga |
852 |
| |
|
R |
caaagccagaggctggtact |
|
|
|
R |
cctctcacagggttgtaagga |
|
| |
2 |
F |
agaggagaaatgcaggctca |
600 |
GJA3 |
1 |
1F |
ttgtgtagtgcctgctcgtc |
711 |
| |
|
R |
gcagacaggagcaagggtag |
|
|
|
1R |
agctcgaagccgtacagaaa |
|
| |
3 |
F |
atggaaattggcaaacgcta |
586 |
|
|
2F |
aaagagagggaggaggagga |
668 |
| |
|
R |
tcctggtccccagacctcca |
|
|
|
2R |
gcccagttctgctcagtcat |
|
| |
4 |
F |
tagacacgtagtgggtgcac |
405 |
|
|
3F |
gctggaagaagctcaagcag |
803 |
| |
|
R |
cagaggtcagcagagcacac |
|
|
|
3R |
aagcattgaacacggaaacc |
|
| |
5 |
F |
tatcacccccttgctctgac |
1105 |
GJA8 |
1 |
1F |
tctgcacaaaggaagcactg |
690 |
| |
|
R |
cccctgagagtgactgtgct |
|
|
|
1R |
cggaacccgtacaggaagta |
|
|
CRYBB3 |
1 |
F |
gagcctcagagttcccctct |
512 |
|
|
2F |
gtgctgcagatcatcttcgt |
825 |
| |
|
R |
gcagcaaagtcatgaagcaa |
|
|
|
2R |
tgcttcctccttcttctctcc |
|
| |
2 |
F |
tgaagttcctgaaggcgttt |
501 |
|
|
3F |
tgagaaatccctccactcca |
752 |
| R | aggtatcctgggattttctgc | 3R | gtagcccttatgctggatgc |
The letters F and R indicate forward and reverse primers, respectively.