Table 1. Primer sequences and annealing temperature used to PCR amplifications of COL4A1 and COL4A2 fragments.
| Name | Forward | Reverse | Annealing Temperature (°C) | Amplicon Size (bp) |
|---|---|---|---|---|
| COL4A1.1 |
CACCCTCCCCCTTTCTACTC |
GCCCAGAGAATGCACCTG |
59 |
837 |
| COL4A1.2 |
TTGGGCTGAGTAACACTTGG |
GCCTGGTTTGGCTTCATTTG |
58 |
459 |
| COL4A1.3–4 |
GGGCAACAGAATGAGACTCC |
TGTGAGCTGGGAGAGGAGAT |
66 |
477 |
| COL4A1.5–6 |
TGCTCTGTCTGCTTTGTGTG |
ACAAGCTGTGCTACTGGGTA |
60 |
698 |
| COL4A1.7–8 |
CCAACAAATGAAGGGTAGGG |
TGTGCCAAGTGTCTGAACG |
58 |
578 |
| COL4A1.9–10 |
CCTTTGCTTTGCCGTCTCTA |
TCATCATCCCTTTCCCACAG |
60 |
691 |
| COL4A1.11 |
GGAGATGGATTGGTATTGGT |
GACTAAGGGATGGATGAAAG |
58 |
451 |
| COL4A1.12 |
GGGACAAAGCTATTGCCTGA |
GACATTGATCCAAAGGTGGG |
58 |
239 |
| COL4A1.13 |
GCAGAGGCAAGGATGATTAG |
GGGGCTCGTATTTTATGGAC |
58 |
393 |
| COL4A1.14–15 |
CCCTGCCCTGCTTACATT |
GTCCCTACGAGCCTTTTCTG |
60 |
505 |
| COL4A1.16–17 |
TTAGTGGAGACGGGATTTCG |
AACTGCCTGCTTGTGTATGC |
60 |
725 |
| COL4A1.18 |
GATGGGACAAGTATCTGGGC |
CATCTCCTCTCCTTCCTCTC |
60 |
459 |
| COL4A1.19 |
GCTACCATTGCTGCTACTTCAC |
AATAGAAAGCGTGGGGAGAG |
62 |
447 |
| COL4A1.20 |
GTCACAACAGGCTTCAGGAG |
CCCAGGAGAGACATAAGGGT |
60 |
486 |
| COL4A1.21 |
CAGTGATGGTCTGGTTGGAT |
ATGCCAGGAGTCTCAGAGGT |
60 |
532 |
| COL4A1.22 |
TGGGTGGTGTGTGGTGATTA |
GAGAAGGGGCAAAACTCTGA |
60 |
516 |
| COL4A1.23 |
TTCCACCCATTAGCAGAGAG |
GCCAACACACCAAAGCAA |
60 |
304 |
| COL4A1.24 |
GTCCGTCTTGGGCATTTTAG |
ATTTGGGCTCTGTGGGTAAC |
60 |
431 |
| COL4A1.25 |
GTGCCCAAAGCCACACTATA |
TGTTCAGTTCCCCCAAATGC |
60 |
718 |
| COL4A1.26 |
CCTGGGAGGGTAGATGAAGT |
GAAAGGGAGGCACAAAAGG |
62 |
488 |
| COL4A1.27–28 |
AAGTGGAGAACACAGGCAGA |
TCTTCCCAACCAAACCCTAC |
56 |
636 |
| COL4A1.29 |
AGGTGCTGGAAGAGACAGCA |
GCTGAGGCTGAGAAACCATC |
60 |
678 |
| COL4A1.30 |
GCTTGAAAAGGGTTGAGCAG |
GGCCTCTAAGATTTGCATCG |
64 |
315 |
| COL4A1.31 |
CAGAGCCCCTACCGAGTATA |
CAGTGGGTGGGAGAAGAATC |
61 |
483 |
| COL4A1.32–33 |
CATTCAAGTTCCCAGTGTGG |
GCCTTCTGCTTGATGTTCCT |
60 |
653 |
| COL4A1.34 |
CTCATTTACCTGGGGTTGGA |
TATGGAGGACCCGATAACCC |
60 |
411 |
| COL4A1.35–36 |
TGTGCCTTTCCTGGGTTATC |
AATGTCATCCATCCCTGAGC |
64 |
594 |
| COL4A1.37 |
GGGGGATTCACGTTCTTGTA |
TCCCTGTGTGTTATGGCTCA |
58 |
364 |
| COL4A1.38–39 |
TGGCAGGTAGAAACCAGATG |
TGAAGATGGGAGACAGGACA |
61 |
641 |
| COL4A1.40 |
GACCTCAGGAAAACCAGGTG |
GTAGTTGCAGGGATGTGCAG |
60 |
359 |
| COL4A1.41 |
TGGTGGTTCTGAGCTGAAAG |
CATGTGTCTTGCAGGCATTG |
60 |
447 |
| COL4A1.42 |
TAAAGAGAAGGAGGGATCGG |
TCTTCACCAGAACCCACAAG |
60 |
673 |
| COL4A1.43 |
CCTGCCTCGATTTCTGTCTC |
TAGTGGGGATGTGGGAGTGT |
60 |
435 |
| COL4A1.44 |
CCACAAGGCACCATTTGTTC |
TACAAATTGGGCTGCCACAC |
60 |
376 |
| COL4A1.45 |
GGACCAAAAACAGTGCCCTA |
GAGCCTTGGGAAGTTTCTGA |
60 |
790 |
| COL4A1.46 |
CCAGAATGCAGTGGGAAGTT |
TTCCTGGGTTTTCTTCTGGA |
60 |
590 |
| COL4A1.47 |
ACAGCAAGAAACCAGGGAGA |
GGCTGCCTTTCAACAACATC |
60 |
591 |
| COL4A1.48 |
TGAAGGAGGTAGGCTGCTGT |
CGCAGTGTTTCACTCGCTAC |
60 |
516 |
| COL4A1.49 |
TGTTGTGAAAGACATTGCCC |
GCCCAGCCAACTGACTTTTA |
60 |
650 |
| COL4A1.50 |
AAAACCAACGGGGAGGTACT |
TAAGCAGCGAGATGCAGAGA |
59 |
407 |
| COL4A1.51 |
GGAAGCAGCCATTAGACGAT |
AAATCGTCTCGGTCATCTGC |
60 |
573 |
| COL4A1.52.1 |
TACCAGGTTGAGGCCTGATG |
ACCTCCTAGCACCCTTTGGT |
65 |
530 |
| COL4A1.52.2 |
GAAAACCAAAGGGTGCTAGG |
CCGAATGTGCTTACGTGTGA |
65 |
793 |
| COL4A1.52.3 |
CCTGGCTTGAAAAACAGCTC |
AATCACCCCCAGTCTGTGAC |
60 |
429 |
| COL4A2.1 |
TCGTGGGAAAGCTCAGATAC |
AGACAAAGCGAGTTTAGCGC |
60 |
1454 |
| COL4A2.2 |
GCTTCTGGAAGGGCCAAT |
GGGAAAGGGAGGAAGAGAGA |
60 |
587 |
| COL4A2.3 |
CCTCATCCTGCGCTAAACTC |
ACACTTTCCTGGCCTCTACG |
60 |
625 |
| COL4A2.4 |
ATTTCAGGGGTGGGAGAGAC |
CGGCCATCTAGGTTTGTGTG |
60 |
467 |
| COL4A2.5–6 |
TTCTTTCATCCCAACCCAGT |
TCCCCACGTGTTTTATGTCA |
59 |
663 |
| COL4A2.7 |
AGACAGAAGAAACCCCGACA |
TCTTGGGCGTCAACATACAG |
60 |
515 |
| COL4A2.8 |
TCAGAATAACCCCCATCAGC |
AACAGATCAGCCCTATCAGGAC |
60 |
568 |
| COL4A2.9–10 |
AGGTCCTGATAGGGCTGATCT |
TAACTGGCAGAGAGCTGGTG |
59 |
551 |
| COL4A2.11 |
GCATCAGAAACCTCCATGC |
ACATTGGCCTCCCTACAACA |
59 |
556 |
| COL4A2.12 |
TCCAATCTCAGCTCCCACTC |
TGTCCTCACCTCCACCTTCT |
60 |
548 |
| COL4A2.13 |
GGAAACAACCCCACAGAAAC |
GGAGGACCCGGTTATGTTTT |
59 |
524 |
| COL4A2.14 |
GTAAACATCTGCCTGGAACG |
CTATGGACAAGGGGATGAGA |
58 |
469 |
| COL4A2.15 |
TGTCACTGCCTGTCCTCAGA |
CCCCAGTGCTAGATGTTCGT |
61 |
513 |
| COL4A2.16 |
ATTATTTCCCATCCCCACCT |
GCAAAAATGAGAGCCAAGGT |
59 |
473 |
| COL4A2.17 |
CCCAGTGTCTTCAACAACCA |
TGTCAGAGGCCGTGTATTTG |
59 |
505 |
| COL4A2.18 |
AGCACAGTCTCCTGGCATTC |
CAGGCAACATGAAGGTCTCC |
60 |
569 |
| COL4A2.19 |
TTCGAGCTTTGGACTCACCT |
CTGTGAAGGTGTCCAAAGCA |
60 |
521 |
| COL4A2.20 |
ACCCATCGGAGTTATTGACG |
TACAGGGCTTCAGCTTCCAT |
60 |
490 |
| COL4A2.21 |
CCTGCATCTGTGGTTGTCTC |
AAGTTCGCCTCCTCATCAAC |
59 |
609 |
| COL4A2.22 |
CCTCTGAATGTGGTCCCAGT |
AAAGTCCGCCTTGGGGTAT |
59 |
602 |
| COL4A2.23–24 |
ATCGCAGAAAGTGCTCCTTG |
ATGAGCAGCCTGTCCTATGC |
60 |
545 |
| COL4A2.25 |
TGGCACTAGGTTCCTGTTCA |
ACAGGAGAGGCTGCATGTTT |
59 |
553 |
| COL4A2.26 |
AAACATGCAGCCTCTCCTGT |
TTCTGACAAGAGGGGTTTGG |
60 |
492 |
| COL4A2.27 |
CCAGAATGGTAGCCGGTTT |
GCAAGACCAGTTTGTGCTGA |
60 |
318 |
| COL4A2.28 |
TAAGCCTGGAGGTGCTGTTT |
CCGAAACACCTGTCTCCTTT |
59 |
499 |
| COL4A2.29 |
GCGAAGGTTGTAGGTTCCAA |
TGCCAAGACAAACAGTGAGC |
60 |
708 |
| COL4A2.30 |
GAATAGACAAGGGCAGGAAGG |
CAGAGGATGAGCCGATGTCT |
60 |
581 |
| COL4A2.31 |
CACAGCCTCAACCTCCAGAT |
CAGGCAGGAGCAGTTTGTCT |
60 |
643 |
| COL4A2.32 |
TGCTCCTCTGCCTTTGTCTT |
TGTTGAGGCAGGGATAAAGC |
60 |
656 |
| COL4A2.33 |
TGGTCTCTCTCCAAGGCTTC |
ACCGAGGTTACTCAGGCATC |
59 |
442 |
| COL4A2.34 |
ACAGCACGTAGGACAGCAAA |
ACATCTGCATGGTGTCCAAG |
59 |
470 |
| COL4A2.35 |
GCTAAGCAAACCGCCTATGA |
ACAGGACTTTCCACTGGGACT |
60 |
416 |
| COL4A2.36 |
GGGAGTCCACAATTCAGAGC |
GACCCTTCGCTGTTTCTGAG |
59 |
629 |
| COL4A2.37 |
CCCATGCTTCTCTCCAATTC |
ATGCCTCTCTCCATTCCTGA |
60 |
446 |
| COL4A2.38 |
CTGCTGCTGCTTTCTGTGTT |
CCTGTGCTGCTATGTTGGTG |
60 |
626 |
| COL4A2.39 |
GTGCTGTCCCACACATGAAA |
AGTCCATTCAACCCAGCAAC |
61 |
510 |
| COL4A2.40 |
ATGGGCCTCGATCCTCTTAT |
AAACCAGCTCTTTCCTGCAC |
60 |
484 |
| COL4A2.41 |
CCCACCATGAGATGTTCCTT |
ATGACACAGGAGGAGCCATC |
60 |
427 |
| COL4A2.42–43 |
AGTCATTCCATGCCACAGAC |
TAAGCTCTCCATTCCCCAAG |
60 |
666 |
| COL4A2.44–45 |
CCCGTTAGTGTCTGGCTCAT |
AGGTGTTCTGCTGGGCATAG |
60 |
744 |
| COL4A2.46 |
GAAACTGCCCTGCACTCCT |
TAGATGGACCCTTCCGTCAG |
60 |
664 |
| COL4A2.47 |
CACTCCCTGGTGATCCAACT |
CCAACTACCCTTGTGCAGTG |
60 |
675 |
| COL4A2.48.1 |
GGATGCCTCATGTCCGTATT |
TACATGGGTGTGTGCGAAGT |
60 |
689 |
| COL4A2.48.2 |
CATCCAGCAGCAGCACTTAG |
AGGTCTCCACTTCTGCCTGA |
59 |
530 |
| COL4A2.48.3 | CCTGCTTTCTACGCCAATGT | CTGGTTGGGGTGTTTTCTGT | 60 | 573 |
In the table, Amplicon Size represents length of the PCR product in base pairs (bp) and Annealing Temperature represents the annealing temperature of the primers used for PCR amplifications.