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. 2011 Apr 25;6(4):e18922. doi: 10.1371/journal.pone.0018922

Figure 6. Effect of Nico (81 mg/l of drinking water) on cardiac disease at late stage in the HSPB5 R120G (R120G) TG mice.

Figure 6

(A) Protocol was shown. Nico was administered via drinking water from 20 weeks of age in the R120G TG mice. (B) Fractional shortening assessed by the echocardiogram. Cardiac functional measurements were made at 28 weeks (n = 6–10 mice). (C) The ratios of heart weight/body weight. (n = 6–10). (D) Representative pictures of the immunohistochemistry are shown. The aggregates containing the mutant R120G protein (upper panel green) and amyloid oligomer (lower panel green) were observed in the R120G TG mice. To distinguish between the cardiomyocytes, cardiac troponin I was stained (upper panel red) (E and F) Quantitative analysis of the aggregates containing the mutant HSPB5 R120G protein (E) and amyloid oligomer (F). (n = 4). (G) Masson's trichrome stain in the R120G TG mouse heart with or without Nico treatment. * p<0.05, *** p<0.001 vs. non-transgenic (NTG) mice; # p<0.05, ### p<0.001 vs. R120G TG mice.