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. 2011 Apr 25;6(4):e18922. doi: 10.1371/journal.pone.0018922

Figure 7. Effect of Nico (81 mg/l of drinking water) on survival rate and biochemical parameters at the late stage in the HSPB5 R120G (R120G) TG mice.

Figure 7

(A) Survival curves. Nico effectively attenuated premature death in R120G TG mice. (B) Typical pictures of Western blot analysis for ANF. (C) Quantitative analysis of ANF in the hearts in the R120G TG mice. (D) Quantitative analysis of TUNEL-positive cardiomyocytes. (E) Typical pictures of Western blot analysis. (F) Quantitative analysis of HSPB5 and. Values are the x-fold increase relative to values in non-transgenic (NTG) mouse hearts whose values are arbitrarily set to 1. (D) Representative pictures of the immunohistochemistry are shown. The aggregates containing the mutant R120G protein are observed in R120G TG mice with or without Nico treatment. (E) Cytochrome c levels of mitochondrial fraction isolated from the R120G TG mouse heart with or without Nico treatment. (F) Typical pictures of Western blot analysis. Quantitative analysis of cytochrome c (F upper colomun) and HSPB5 (F lower column) in mitochondrial and cytosolic fraction isolated from the R120G TG mice. GAPDH was used as a loading control in the cytosolic fraction and voltage-dependent anion channel (VDAC) was used in the mitochondrial fraction. ***p<0.001 vs. non-transgenic (NTG) mice; # p<0.05, ### p<0.001 vs. R120G TG mice (survival rate: n = 19–20, and other experiments: n = 4).