Table 1. RT-PCR primers.
Gene symbol | Tissue ampli-fied | Forward primer | Reverse primer | Expected cDNA size (bp) | Comment |
Actb | Retina, LGN | gaaatcgtgcgtgacatca | aacgcagctcagtaacagt | 535 | |
Chrnb2 | Retina, LGN | gtatcattggcacagctca | gcaatgaagcgtacaccgt | 1120 | |
Pisd-ps3 (Rik4933439C20) | Retina, LGN | ctcttggtggtctttcaag | agaaactctacagacgcca | 270 | Multiple chromosomal loci |
Plac9 | Retina | aggcgactacggacaaact | ttgcacaggtcacccaggt | 590 | |
Ccl21 (6CKine) | Retina | gatgactctgagcctcctt | gtctgttcagttctcttgca | 370 | |
Rik4933409K07 | Retina | caagtctgtgttgacatgga | ttaataatgtacagcagagaca | 715 | |
Crb1 | retina | cttgtgtctgccctcaaga | gtgcagcccaggagaattt | 710 | 4 known isoforms |
Xlr3a | LGN | caattactggttagcacacat | tatccatacaagtgagggat | 420 | |
Spp1 | LGN | agaagcatccttgcttgggt | cttcatgtgagaggtgaggt | 615 | |
Cdh1 | LGN | cccaagttgcccagttct | atcttagagaacggtttcaat | 545 | |
Lypd2 | LGN | ttggcactgatattgggca | ccatggctttacagcagga | 370 |