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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Stem Cell Res. 2011 Jan 19;6(3):195–205. doi: 10.1016/j.scr.2011.01.002

Figure 2. Concentration-dependent effects of BoNT/A on SNAP-25 protein cleavage in mouse ES-derived motoneurons, primary mouse spinal neurons and DRG neurons, and the motoneuron like NSC-34 cell line.

Figure 2

A–D; Graphic representation of the percentage of uncleaved SNAP-25 protein following cell treatment with various concentrations of BoNT/A (0–1000 pM). The graphs represent the quantification of each blot shown at the bottom of the x-axis. The top band is uncleaved SNAP-; the bottom band is the BoNT/A mediated cleavage fragment. Inset in each panel are representative image of : (A) ES-derived motoneuron cultures stained with the neuronal marker NeUN (red), Hb9-eGFP (green) and DAPI (blue); (B) primary mouse spinal cord cells and (C) DRG neurons stained with cytoplasmic neuronal markers Tuj1 (Red) and DAPI (Blue); (D) immortalized NSC-34 cells stained with neuronal marker Tau5 (Red) for total Tau protein and DAPI imaging. The scale bar is 50 µm.