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. Author manuscript; available in PMC: 2012 Jun 29.
Published in final edited form as: Biochemistry. 2010 Dec 29;50(3):388–396. doi: 10.1021/bi101239r

FIGURE 5. Analysis of pore formation activity of Cyt1Aa and its N-terminal and C-terminal fragments using calcein leakage assay.

FIGURE 5

Calcein loaded SUV (PC:Ch:S mixture) suspended in 150 mM KCl, CHES 10 mM, pH 9, were incubated with the different protein samples at two concentrations and the release of calcein was analyzed. Maximal leakage at the end of each experiment was assessed with 0.1% Triton-X-100. Each value represents the mean ±SD of three independent experiments. A t-test was used to analyze statistical differences of mean values of percentage of calcein released by different concentrations of Cyt1Aa or the toxin-fragments when compared with the control (No-toxin). Bars labeled with different letters indicated that differences were statistically significant. Insert shows a representative trace of the calcein release assay performed with Cyt1Aa toxin.