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. Author manuscript; available in PMC: 2012 Jun 29.
Published in final edited form as: Biochemistry. 2010 Dec 29;50(3):388–396. doi: 10.1021/bi101239r

TABLE 1.

Sequence of primers used in PCR assays.

Primer
name
sequence Restriction
site
p20f 5’AAA CCC GGG TTT GAC GAG GAA ACA GAG TAT ACG AGT T3’ XmaI
p20r 5’TTT CCC GGG AAA TCG AAC GTC ATA TAG ATA AAA TGC3’ XmaI
pCytf 5’ACA AAG CTT GGC ATC TTT CGA ACT ATA GC3’ HindIII
pCytr 5’GAT GGT ACC TAT GAA AAT ATA ACG TTG3’ KpnI
pNT1r 5’CTA AGA TTA GTA ATT TGT TTG ATT AGC AGT TTC CTT3’ none
pNT2f 5’GAA ACT GCT AAT CAA ACA AAT TAC TAA TCT TAG3’ none
pCT1r 5’GAC ATT GTA TGT GTA ATT TGT CAT AAA TAA ACA ACT CCT3’ none
pCT2f 5’AGG AGT TGT TTA TTT ATG ACA AAT TAC ACA TAC3’ none