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Effects of Cu2+ binding on Bis-ANS fluorescence of mini-αA-crystallin. (A) Quenching of mini-αA bound Bis-ANS fluorescence by Cu2+ binding. (B) Bis-ANS fluorescence intensity in the presence of mini-αA-crystallin and increasing concentrations of Cu2+. The extent of bis-ANS quenching (F0-F) is calculated where F0 and F emission maxima at 490nm are shown in the absence and presence of Cu2+. The inset shows semi log plot of F0-F/F0 vs Cu2+ concentration.
