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. Author manuscript; available in PMC: 2012 Apr 10.

Published in final edited form as: J Control Release. 2010 Dec 28;151(1):95–101. doi: 10.1016/j.jconrel.2010.12.011

Evaluation of the gene silencing by the biopolymers. A) SKOV3/GFP cells were transfected with GFP-siRNA alone, FDNT/GFP-siRNA and FDT/GFP-siRNA to knock down GFP expression. 1:5 ratio refers to 1 ug of GFP-siRNA to 5 ug of biopolymer. Scrambled siRNA was used as a control. The level of GFP expression was measured using flowcytometry. B) Epifluorescent images of the untreated GFP̄ and GFP⁺ cells as well as SKOV3/GFP cells treated with biopolymers in complex with GFP-siRNA. C) Knockdown of BCL2 protein expression in SKOV3/BCL2 cancer cells that overexpress BCL2. BCL2-siRNA was complexed with FDT to knockdown the BCL2 expression. i) Knockdown was detected by westernblot analysis using anti-BCL2 antibody. Lane 1: BCL2 protein (positive control); Lane 2: FDT/BCL2-siRNA (1:1 ratio); Lane 3: FDT/BCL2-siRNA (1:5 ratio); Lane 4: scrambled-siRNA (1:5 ratio); ii) GAPDH control.

Evaluation of the gene silencing by the biopolymers. A) SKOV3/GFP cells were transfected with GFP-siRNA alone, FDNT/GFP-siRNA and FDT/GFP-siRNA to knock down GFP expression. 1:5 ratio refers to 1 ug of GFP-siRNA to 5 ug of biopolymer. Scrambled siRNA was used as a control. The level of GFP expression was measured using flowcytometry. B) Epifluorescent images of the untreated GFP̄ and GFP⁺ cells as well as SKOV3/GFP cells treated with biopolymers in complex with GFP-siRNA. C) Knockdown of BCL2 protein expression in SKOV3/BCL2 cancer cells that overexpress BCL2. BCL2-siRNA was complexed with FDT to knockdown the BCL2 expression. i) Knockdown was detected by westernblot analysis using anti-BCL2 antibody. Lane 1: BCL2 protein (positive control); Lane 2: FDT/BCL2-siRNA (1:1 ratio); Lane 3: FDT/BCL2-siRNA (1:5 ratio); Lane 4: scrambled-siRNA (1:5 ratio); ii) GAPDH control.