Figure 1.
Diagrams depict endpoints analyzed for densitometry and morphology. (A) Schematics represent the regions sampled in a level of the rostral LC (left) and a middle level of the rostrocaudal LC (right). The rostral and mid LC regions are AP −9.30 and −9.80 relative to Bregma, respectively [62]. The nuclear core (i.e., LC cell bodies) is defined by the dashed line. The double line demarcates the area of the ventromedial dendrites analyzed. The shaded triangle represents the region of interest used to assess the density of the ventromedial dendrites. The striped triangle represents the region of interested used to assess the density of the dorsolateral dendrites. (B) Shown on the representative trace are examples of an end, a node, and segments. This neuron also had primary (1st), secondary (2nd), and tertiary dendrites (3rd). Abbreviations: IVth, fourth ventricle; D, dorsal; DL, dorsolateral; M, medial; VM, ventromedial