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(A) Near UV-Vis spectrum of apo-hRXRα-LBD homodimer (black), hRXRα-LBD:9cRA complex in the absence of GRIP-1 (Red), and hRXRα-LBD:9cRA:GRIP-1 complex (Blue). (B) Difference UV-Vis spectrum of hRXRα-LBD:9cRA complex (Red) or the hRXRα-LBD:9cRA:GRIP-1 complex (Blue) minus the spectrum of apo-hRXRα-LBD. (C). Difference UV-Vis spectrum of hRXRα-LBD:9cRA:GRIP-1 minus the spectrum of hRXRα-LBD:9cRA (Black) versus the computer-fitted difference spectrum (Green).
