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. Author manuscript; available in PMC: 2012 Jun 8.
Published in final edited form as: Biochemistry. 2010 Dec 8;50(1):93–105. doi: 10.1021/bi101288y

Figure 6.

Figure 6

HDX MS analyses mapped onto hRXRα-LBD:9cRA:GRIP-1. Regions within the hRXRα-LBD that do not change deuterium incorporation with 9cRA or 9cRA + GRIP-1 are colored in grey. Upon 9cRA binding, several HDX MS monitored peptides (yellow and orange) around the ligand binding pocket of hRXRα-LBD become significantly more protected from deuterium incorporation (10–37% change relative to hRXRα-LBD, see Table 3). When GRIP-1 (green) is added to the complex, a subset of these same regions (in orange, H3, H4, H10, and H7) become further protected or stabilized (>7% additional change relative to hRXRα-LBD:9cRA) demonstrating the stabilizing affect of GRIP-1 binding on the hRXRα-LBD beyond the coactivator binding site. Signficantly, several peptides that are part of H12 and the C-terminal end of H11 (in red) were not protected (remained dynamic) by 9cRA addition but then did show protection when GRIP-1 is added to the hRXRα-LBD:9cRA complex.