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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2010 Dec 24;127(6):1400–1407.e4. doi: 10.1016/j.jaci.2010.11.008

Figure 3. Differentiation of PID-specific iPSCs reveals lineage-specific gene expression.

Figure 3

PID-specific iPSC were allowed to differentiate into embryoid bodies (EB) by culture in a bFGF-free hES medium and without co-culture with feeder cells. Robust formation of tight and well-formed cell clusters was detected by day 7, that became cystic by day 10 (upper row in each cell-specific panel). Quantitative RT-PCR gene expression analysis of the derived EB after 10 days shows increased expression of lineage-specific markers from each of the three embryonic germ layers, including: AFP and GATA4 (endoderm), RUNX1 and Brachyury (mesoderm), NCAM and NESTIN (ectoderm). Quantitative RT-PCR reactions were normalized against β-actin (ACTB). Expression was calculated using the ddCT method relative to expression levels in undifferentiated iPSCs. Black and white bars identify undifferentiated iPSCs and day 10 EB, respectively.